Deciphering mouse uterine receptivity for embryo implantation at single-cell resolution.

ABSTRACT:

Objectives

Mice are widely used as an animal model for studying human uterine receptivity for embryo implantation. Although transcriptional changes related to mouse uterine receptivity have been determined by using bulk RNA-seq, the data are of limited value because the uterus is a complex organ consisting of many cell types. Here, we aimed to decipher mouse uterine receptivity for embryo implantation at single-cell resolution.

Materials and methods

Single-cell RNA sequencing was performed for the pre-receptive and the receptive mouse uterus. Gene expression profiles in luminal epithelium and glandular epithelium were validated by comparing against a published laser capture microdissection (LCM)-coupled microarray dataset.

Results

We revealed 19 distinct cell clusters

SUBMITTER: Yang Y 

PROVIDER: S-EPMC8560620 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature