Surf4 facilitates reprogramming by activating the cellular response to endoplasmic reticulum stress.

ABSTRACT:

Objectives

Maternal factors that are enriched in oocytes have attracted great interest as possible key factors in somatic cell reprogramming. We found that surfeit locus protein 4 (Surf4), a maternal factor, can facilitate the generation of induced pluripotent stem cells (iPSCs) previously, but the mechanism remains elusive.

Materials and methods

In this study, we investigated the function and mechanism of Surf4 in somatic cell reprogramming using a secondary reprogramming system. Alkaline phosphatase (AP) staining, qPCR and immunofluorescence (IF) staining of expression of related markers were used to evaluate efficiency of iPSCs derived from mouse embryonic fibroblasts. Embryoid body and teratoma formation assays were performed to evaluate the differentiation ability of th

SUBMITTER: Wu L 

PROVIDER: S-EPMC8560622 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature