Development of surface engineered antigenic exosomes as vaccines for respiratory syncytial virus.

ABSTRACT: Respiratory syncytial virus (RSV) is one of the main pathogens associated with lower respiratory tract infections in infants and young children worldwide. Exosomes secreted by antigen presenting cells (APCs) can elicit immune responses by carrying major histocompatibility complex (MHC) class I molecules complexed with antigenic peptides and other co-stimulating factors. Therefore, we developed novel immunomagnetic nanographene particles to sequentially isolate, surface engineer, and release intact dendritic cell (DC) exosomes for use as a potential vaccine platform against RSV. The H-2D^b-restricted, immunodominant peptides from RSV (M_187-195 and NS1_61-75) were introduced to MHC-I on DC-derived exosomes to express peptide/MHC-I (pMHC-I) complexes. A mouse mo

SUBMITTER: Hong S 

PROVIDER: S-EPMC8560785 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature