Ablation of mitochondrial DNA results in widespread remodeling of the mitochondrial complexome.

ABSTRACT: So-called ρ0 cells lack mitochondrial DNA and are therefore incapable of aerobic ATP synthesis. How cells adapt to survive ablation of oxidative phosphorylation remains poorly understood. Complexome profiling analysis of ρ0 cells covered 1,002 mitochondrial proteins and revealed changes in abundance and organization of numerous multiprotein complexes including previously not described assemblies. Beyond multiple subassemblies of complexes that would normally contain components encoded by mitochondrial DNA, we observed widespread reorganization of the complexome. This included distinct changes in the expression pattern of adenine nucleotide carrier isoforms, other mitochondrial transporters, and components of the protein import machinery. Remarkably, ablation of mitochondrial DNA hardly aff

SUBMITTER: Guerrero-Castillo S 

PROVIDER: S-EPMC8561636 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature