Molecular diversity of diencephalic astrocytes reveals adult astrogenesis regulated by Smad4.

ABSTRACT: Astrocytes regulate brain-wide functions and also show region-specific differences, but little is known about how general and region-specific functions are aligned at the single-cell level. To explore this, we isolated adult mouse diencephalic astrocytes by ACSA-2-mediated magnetic-activated cell sorting (MACS). Single-cell RNA-seq revealed 7 gene expression clusters of astrocytes, with 4 forming a supercluster. Within the supercluster, cells differed by gene expression related to ion homeostasis or metabolism, with the former sharing gene expression with other regions and the latter being restricted to specific regions. All clusters showed expression of proliferation-related genes, and proliferation of diencephalic astrocytes was confirmed by immunostaining. Clonal analysis demonstrated l

SUBMITTER: Ohlig S 

PROVIDER: S-EPMC8561644 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature