Mechanistic Inferences From Analysis of Measurements of Protein Phase Transitions in Live Cells.

ABSTRACT: The combination of phase separation and disorder-to-order transitions can give rise to ordered, semi-crystalline fibrillar assemblies that underlie prion phenomena namely, the non-Mendelian transfer of information across cells. Recently, a method known as Distributed Amphifluoric Förster Resonance Energy Transfer (DAmFRET) was developed to study the convolution of phase separation and disorder-to-order transitions in live cells. In this assay, a protein of interest is expressed to a broad range of concentrations and the acquisition of local density and order, measured by changes in FRET, is used to map phase transitions for different proteins. The high-throughput nature of this assay affords the promise of uncovering sequence-to-phase behavior relationships in live cells. Here, we report t

SUBMITTER: Posey AE 

PROVIDER: S-EPMC8561728 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature