Crystal structure of acetylxylan esterase from Caldanaerobacter subterraneus subsp. tengcongensis.

ABSTRACT: The acetylxylan esterases (AXEs) classified into carbohydrate esterase family 4 (CE4) are metalloenzymes that catalyze the deacetylation of acetylated carbohydrates. AXE from Caldanaerobacter subterraneus subsp. tengcongensis (TTE0866), which belongs to CE4, is composed of three parts: a signal sequence (residues 1-22), an N-terminal region (NTR; residues 23-135) and a catalytic domain (residues 136-324). TTE0866 catalyzes the deacetylation of highly substituted cellulose acetate and is expected to be useful for industrial applications in the reuse of resources. In this study, the crystal structure of TTE0866 (residues 23-324) was successfully determined. The crystal diffracted to 1.9 Å resolution and belonged to space group I2₁2₁2₁. The catalytic domain (r

SUBMITTER: Sasamoto K 

PROVIDER: S-EPMC8561816 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature