Protein profile of fiber types in human skeletal muscle: a single-fiber proteomics study.

ABSTRACT:

Background

Human skeletal muscle is composed of three major fiber types, referred to as type 1, 2A, and 2X fibers. This heterogeneous cellular composition complicates the interpretation of studies based on whole skeletal muscle lysate. A single-fiber proteomics approach is required to obtain a fiber-type resolved quantitative information on skeletal muscle pathophysiology.

Methods

Single fibers were dissected from vastus lateralis muscle biopsies of young adult males and processed for mass spectrometry-based single-fiber proteomics. We provide and analyze a resource dataset based on relatively pure fibers, containing at least 80% of either MYH7 (marker of slow type 1 fibers), MYH2 (marker of fast 2A fibers), or MYH1 (marker of fast 2X fibers).

Results

In a dataset of

SUBMITTER: Murgia M 

PROVIDER: S-EPMC8561870 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature