Transforming growth factor-β1 decreases erythropoietin production through repressing hypoxia-inducible factor 2α in erythropoietin-producing cells.

ABSTRACT:

Background

Renal erythropoietin (EPO)-producing (REP) cells produce EPO through hypoxia-inducible factor (HIF) 2α-activated gene transcription. Insufficient EPO production leads to anemia in patients with chronic kidney disease. Although recombinant EPO is effective to improve anemia, no reliable REP cell lines limit further progress of research and development of novel treatment.

Methods

We screened Epo mRNA expression in mouse fibroblast cell lines. Small interfering RNA specific for HIF1α or HIF2α was transfected to study Epo expression in C3H10T1/2 cells. The effect of transforming growth factor-β1 (TGF-β1) on HIF-EPO axis was studied in C3H10T1/2 cells and mice.

Results

Similar to mouse REP pericytes, C3H10T1/2 cells differentiated to α-smooth muscle actin^+<

SUBMITTER: Shih HM 

PROVIDER: S-EPMC8561873 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature