Self-extracellular RNA promotes pro-inflammatory response of astrocytes to exogenous and endogenous danger signals.

ABSTRACT:

Objective

Astrocytes participate in the local innate immune response of the central nervous system. In response to stress such as ischemia, activated cells release endogenous factors known as damage-associated molecular patterns (DAMPs). Self-extracellular RNA (eRNA) is such a ubiquitous alarm signal. However, it is unclear whether eRNA is involved in the early acute phase of cerebral ischemia and is sufficient to sensitize astrocytes towards a DAMP or PAMP (pathogen-associated molecular pattern) reaction.

Methods

Pro-inflammatory activation upon eRNA stimulation was characterized in primary murine astrocyte cultures. In vivo, an experimental stroke model was used to localize and quantify eRNA in murine brain sections. Using primary cortical neurons and the mouse hippocampal

SUBMITTER: Fischer S 

PROVIDER: S-EPMC8561902 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature