Instant activation of TRP channels by NH₄ ⁺ promotes neuronal bursting and glutamate spikes in CA1 neurons.

ABSTRACT: Ammonia ( NH4+ ) is a by-product of cell metabolism and may elicit subcellular effects with specific physiological responses. Chronic effects have been implicated in several neurological diseases and attributed to persistent elevation in blood ammonia levels transferred to the brain. In previous studies the activities of neurons and astrocytes have been examined at ammonia concentrations an order of magnitude higher than measured in the blood. The effects developed within several minutes. Here we focused upon acute responses of neurons to ammonia and whether they may occur at much lower doses. To this end, we combined patch-clamp in CA1 neurons with glutamate imaging in hippocampal slices. Particular attention was paid to the Rett syndrome that has been originally attributed to hyperammone

SUBMITTER: Balakrishnan S 

PROVIDER: S-EPMC8562246 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature