Cryo-TEM simulations of amorphous radiation-sensitive samples using multislice wave propagation.

ABSTRACT: Image simulation plays a central role in the development and practice of high-resolution electron microscopy, including transmission electron microscopy of frozen-hydrated specimens (cryo-EM). Simulating images with contrast that matches the contrast observed in experimental images remains challenging, especially for amorphous samples. Current state-of-the-art simulators apply post hoc scaling to approximate empirical solvent contrast, attenuated image intensity due to specimen thickness and amplitude contrast. This practice fails for images that require spatially variable scaling, e.g. simulations of a crowded or cellular environment. Modeling both the signal and the noise accurately is necessary to simulate images of biological specimens with contrast that is correct on an

SUBMITTER: Himes B 

PROVIDER: S-EPMC8562658 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature