QCM-D assay for quantifying the swelling, biodegradation, and protein adsorption of intelligent nanogels.

ABSTRACT: Environmentally responsive nanomaterials have been developed for drug delivery applications, in an effort to target and accumulate therapeutic agents at sites of disease. Within a biological system, these nanomaterials will experience diverse conditions which encompass a variety of solute identities and concentrations. In this study, we developed a new quartz crystal microbalance with dissipation (QCM-D) assay, which enabled the quantitative analysis of nanogel swelling, protein adsorption, and biodegradation in a single experiment. As a proof of concept, we employed this assay to characterize non-degradable and biodegradable poly(acrylamide-co-methacrylic acid) nanogels. We compared the QCM-D results to those obtained by dynamic light scattering to highlight the advantages and limitations

SUBMITTER: Clegg JR 

PROVIDER: S-EPMC8562820 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature