Electrogeneration of a Free-Standing Cytochrome c-Silica Matrix at a Soft Electrified Interface.

ABSTRACT: Interactions of a protein with a solid-liquid or a liquid-liquid interface may destabilize its conformation and hence result in a loss of biological activity. We propose here a method for the immobilization of proteins at an electrified liquid-liquid interface. Cytochrome c (Cyt c) is encapsulated in a silica matrix through an electrochemical process at an electrified liquid-liquid interface. Silica condensation is triggered by the interfacial transfer of cationic surfactant, cetyltrimethylammonium, at the lower end of the interfacial potential window. Cyt c is then adsorbed on the previously electrodeposited silica layer, when the interfacial potential, Δ_o^wϕ, is at the positive end of the potential window. By cycling of the potential window back and forth, silica ele

SUBMITTER: Gamero-Quijano A 

PROVIDER: S-EPMC8562870 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature