Candidate host epigenetic marks predictive for HIV reservoir size, responsiveness to latency reversal, and viral rebound.

ABSTRACT:

Objective

This study aimed to identify candidate host epigenetic biomarkers predicting latency reversal agents (LRA) efficacy and HIV-1 rebound kinetics during analytical treatment interruption (ATI).

Design

Retrospective longitudinal epigenetic profiling study from 13 people with HIV (PWH) on virologically suppressive antiretroviral therapy (ART) that participated in a LRA (HDAC inhibitor) clinical trial (NCT01680094) and a subsequent optional ATI to monitor for viral recrudescence after ART cessation.

Methods

Genome-wide DNA methylation (DNAm) in purified CD4+ T cells was measured at single-nucleotide resolution using the Infinium MethylationEPIC array. HIV-1 DNA and RNA measures were previously assessed by PCR-based methods and the association of DNAm levels at reg

SUBMITTER: Corley MJ 

PROVIDER: S-EPMC8563431 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature