Loss of 15-lipoxygenase disrupts T_reg differentiation altering their pro-resolving functions.

ABSTRACT: Regulatory T-cells (T_regs) are central in the maintenance of homeostasis and resolution of inflammation. However, the mechanisms that govern their differentiation and function are not completely understood. Herein, we demonstrate a central role for the lipid mediator biosynthetic enzyme 15-lipoxygenase (ALOX15) in regulating key aspects of T_reg biology. Pharmacological inhibition or genetic deletion of ALOX15 in T_regs decreased FOXP3 expression, altered T_reg transcriptional profile and shifted their metabolism. This was linked with an impaired ability of Alox15-deficient cells to exert their pro-resolving actions, including a decrease in their ability to upregulate macrophage efferocytosis and a downregulation of interferon gamma expression in Th

SUBMITTER: Marques RM 

PROVIDER: S-EPMC8563763 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature