Simultaneous expression of MMB-FOXM1 complex components enables efficient bypass of senescence.

ABSTRACT: Cellular senescence is a stable cell cycle arrest that normal cells undergo after a finite number of divisions, in response to a variety of intrinsic and extrinsic stimuli. Although senescence is largely established and maintained by the p53/p21^WAF1/CIP1 and pRB/p16^INK4A tumour suppressor pathways, the downstream targets responsible for the stability of the growth arrest are not known. We have employed a stable senescence bypass assay in conditionally immortalised human breast fibroblasts (CL3^EcoR) to investigate the role of the DREAM complex and its associated components in senescence. DREAM is a multi-subunit complex comprised of the MuvB core, containing LIN9, LIN37, LIN52, LIN54, and RBBP4, that when bound to p130, an RB1 like protein, and E2F4 inhibits

SUBMITTER: Kumari R 

PROVIDER: S-EPMC8563780 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature