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Short high fat diet triggers reversible and region specific effects in DCX⁺ hippocampal immature neurons of adolescent male mice.

ABSTRACT: Adolescence represents a crucial period for maturation of brain structures involved in cognition. Early in life unhealthy dietary patterns are associated with inferior cognitive outcomes at later ages; conversely, healthy diet is associated with better cognitive results. In this study we analyzed the effects of a short period of hypercaloric diet on newborn hippocampal doublecortin⁺ (DCX) immature neurons in adolescent mice. Male mice received high fat diet (HFD) or control low fat diet (LFD) from the 5th week of age for 1 or 2 weeks, or 1 week HFD followed by 1 week LFD. After diet supply, mice were either perfused for immunohistochemical (IHC) analysis or their hippocampi were dissected for biochemical assays. Detailed morphometric analysis was performed in DCX⁺ cells that displayed features of immature neurons. We report that 1 week-HFD was sufficient to dramatically reduce dendritic tree complexity of DCX⁺ cells. This effect occurred specifically in dorsal and not ventral hippocampus and correlated with reduced BDNF expression levels in dorsal hippocampus. Both structural and biochemical changes were reversed by a return to LFD. Altogether these studies increase our current knowledge on potential consequences of hypercaloric diet on brain and in particular on dorsal hippocampal neuroplasticity.

SUBMITTER: Chiazza F

PROVIDER: S-EPMC8563989 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature

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Short high fat diet triggers reversible and region specific effects in DCX<sup>+</sup> hippocampal immature neurons of adolescent male mice.

Chiazza Fausto F Bondi Heather H Masante Irene I Ugazio Federico F Bortolotto Valeria V Canonico Pier Luigi PL Grilli Mariagrazia M

Scientific reports 20211102 1

Adolescence represents a crucial period for maturation of brain structures involved in cognition. Early in life unhealthy dietary patterns are associated with inferior cognitive outcomes at later ages; conversely, healthy diet is associated with better cognitive results. In this study we analyzed the effects of a short period of hypercaloric diet on newborn hippocampal doublecortin<sup>+</sup> (DCX) immature neurons in adolescent mice. Male mice received high fat diet (HFD) or control low fat di ...[more]

PMID: 34728755

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Dataset Information

Short high fat diet triggers reversible and region specific effects in DCX⁺ hippocampal immature neurons of adolescent male mice.

Publications

Short high fat diet triggers reversible and region specific effects in DCX<sup>+</sup> hippocampal immature neurons of adolescent male mice.

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Dataset Information

Short high fat diet triggers reversible and region specific effects in DCX+ hippocampal immature neurons of adolescent male mice.

Publications

Short high fat diet triggers reversible and region specific effects in DCX<sup>+</sup> hippocampal immature neurons of adolescent male mice.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

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Short high fat diet triggers reversible and region specific effects in DCX⁺ hippocampal immature neurons of adolescent male mice.