Engineering Production of a Novel Diterpene Synthase Precursor in Nicotiana benthamiana.

ABSTRACT: Diterpene biosynthesis commonly originates with the methylerythritol phosphate (MEP) pathway in chloroplasts, leading to the C₂₀ substrate, geranylgeranyl pyrophosphate (GGPP). The previous work demonstrated that over-expression of genes responsible for the first and last steps in the MEP pathway in combination with GERANYLGERANYL PYROPHOSPHATE SYNTHASE (GGPPS) and CASBENE SYNTHASE (CAS) is optimal for increasing flux through to casbene in Nicotiana benthamiana. When the gene responsible for the last step in the MEP pathway, 4-HYDROXY-3-METHYLBUT-2-ENYL DIPHOSPHATE REDUCTASE (HDR), is removed from this combination, casbene is still produced but at lower amounts. Here, we report the unexpected finding that this reduced gene combina

SUBMITTER: Forestier ECF 

PROVIDER: S-EPMC8564105 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature