Selection and validation of reference genes for qRT-PCR analysis during fruit ripening of red pitaya (Hylocereus polyrhizus).

ABSTRACT: Red pitaya (Hylocereus polyrhizus) is widely cultivated in southern and southwestern China. To provide a basis for studying the molecular mechanisms of the ripening of this fruit, we carried out RNA sequencing (RNA-seq) analysis to identify differentially and stably expressed unigenes. The latter may serve as a resource of potential reference genes for normalization of target gene expression determined using quantitative real-time PCR (qRT-PCR). We selected 11 candidate reference genes from our RNA-seq analysis of red pitaya fruit ripening (ACT7, EF-1α, IF-4α, PTBP, PP2A, EF2, Hsp70, GAPDH, DNAJ, TUB and CYP), as well as β-ACT, which has been used as a reference gene for pitayas in previous studies. We then comprehensively evaluated their expression stability during fruit ripening using fo

SUBMITTER: Zheng Q 

PROVIDER: S-EPMC8564333 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature