Molecular Flexibility of Antibodies Preserved Even in the Dense Phase after Macroscopic Phase Separation.

ABSTRACT: Antibody therapies are typically based on high-concentration formulations that need to be administered subcutaneously. These conditions induce several challenges, inter alia a viscosity suitable for injection, sufficient solution stability, and preservation of molecular function. To obtain systematic insights into the molecular factors, we study the dynamics on the molecular level under strongly varying solution conditions. In particular, we use solutions of antibodies with poly(ethylene glycol), in which simple cooling from room temperature to freezing temperatures induces a transition from a well-dispersed solution into a phase-separated and macroscopically arrested system. Using quasi-elastic neutron scattering during in situ cooling ramps and in prethermalized measurements, we o

SUBMITTER: Girelli A 

PROVIDER: S-EPMC8564753 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature