Knockdown of circSMAD2 inhibits the tumorigenesis of gallbladder cancer through binding with eIF4A3.

ABSTRACT:

Background

Gallbladder cancer (GBC) is the seventh most common gastrointestinal cancer worldwide. This study aimed to investigate the function of circSMAD2 in GBC.

Methods

To investigate the function of circSMAD2 in GBC, the level of circSMAD2 in GBC cells was detected by RT-qPCR. CCK-8 assay was performed to investigate the cell viability. Cell apoptosis was tested by flow cytometry. In addition, transwell assay was used to detect the cell migration and invasion. RIP and RNA pull-down were used to explore the relation among circSMAD2, eIF4A3 and SMAD2. Meanwhile, xenograft mice model was established to investigate the function of circSMAD2 in GBC.

Results

The data revealed that circSMAD2 was upregulated in GBC, and circSMAD2 knockdown significantly inhibited the viab

SUBMITTER: Qin Y 

PROVIDER: S-EPMC8564960 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature