Effect of extraction method on the chemical profiles and bioactivities of soybean hull polysaccharides.

ABSTRACT: The yields, properties, and bioactivities of polysaccharides extracted by three methods from soybean hulls (SSCPs) were evaluated: hot water extraction (H-SSCP), microwave-assisted ammonium oxalate extraction (A-SSCP), and microwave-assisted sodium citrate extraction (S-SSCP). A-SSCP gave the highest yield of polysaccharides (9.3 ± 0.5%) although all three products had similar physicochemical characteristics and FT-IR spectra. A-SSCP and S-SSCP produced polysaccharides with lower molecular weight distributions and higher total reducing power and scavenging ability for ABTS⁺• and DPPH• free radicals. Furthermore, the effect of SSCPs on carbon tetrachloride (CCl₄)-induced liver injury was investigated in the mice. When compared with H-SSCP and S-SSCP, A-SSCP significantly decreased the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), malondialdehyde (MDA), and reactive oxygen (ROS) to normal (p < .05) and increased the level of glutathione (GSH) to normal (p < .05). A-SSCP was the most effective polysaccharide, yielding an approximately normal hepatic appearance with well-preserved cytoplasm, obvious cell boundaries, with legible nuclei and nucleoli. This study indicates that polysaccharides extracted from soybean hulls via microwave-assisted ammonium oxalate extraction have the potential to be developed as a new functional food contributing to the alleviation of liver damage.

SUBMITTER: Han L 

PROVIDER: S-EPMC8565245 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature