Modulation of the intrinsic chromatin binding property of HIV-1 integrase by LEDGF/p75.

ABSTRACT: The stable insertion of the retroviral genome into the host chromosomes requires the association between integration complexes and cellular chromatin via the interaction between retroviral integrase and the nucleosomal target DNA. This final association may involve the chromatin-binding properties of both the retroviral integrase and its cellular cofactor LEDGF/p75. To investigate this and better understand the LEDGF/p75-mediated chromatin tethering of HIV-1 integrase, we used a combination of biochemical and chromosome-binding assays. Our study revealed that retroviral integrase has an intrinsic ability to bind and recognize specific chromatin regions in metaphase even in the absence of its cofactor. Furthermore, this integrase chromatin-binding property was modulated by the interaction w

SUBMITTER: Lapaillerie D 

PROVIDER: S-EPMC8565322 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature