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EIF3j facilitates loading of release factors into the ribosome.

ABSTRACT: eIF3j is one of the eukaryotic translation factors originally reported as the labile subunit of the eukaryotic translation initiation factor eIF3. The yeast homolog of this protein, Hcr1, has been implicated in stringent AUG recognition as well as in controlling translation termination and stop codon readthrough. Using a reconstituted mammalian in vitro translation system, we showed that the human protein eIF3j is also important for translation termination. We showed that eIF3j stimulates peptidyl-tRNA hydrolysis induced by a complex of eukaryotic release factors, eRF1-eRF3. Moreover, in combination with the initiation factor eIF3, which also stimulates peptide release, eIF3j activity in translation termination increases. We found that eIF3j interacts with the pre-termination ribosomal complex, and eRF3 destabilises this interaction. In the solution, these proteins bind to each other and to other participants of translation termination, eRF1 and PABP, in the presence of GTP. Using a toe-printing assay, we determined the stage at which eIF3j functions - binding of release factors to the A-site of the ribosome before GTP hydrolysis. Based on these data, we assumed that human eIF3j is involved in the regulation of translation termination by loading release factors into the ribosome.

SUBMITTER: Egorova T

PROVIDER: S-EPMC8565342 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature

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eIF3j facilitates loading of release factors into the ribosome.

Egorova Tatiana T Biziaev Nikita N Shuvalov Alexey A Sokolova Elizaveta E Mukba Sabina S Evmenov Konstantin K Zotova Maria M Kushchenko Artem A Shuvalova Ekaterina E Alkalaeva Elena E

Nucleic acids research 20211101 19

eIF3j is one of the eukaryotic translation factors originally reported as the labile subunit of the eukaryotic translation initiation factor eIF3. The yeast homolog of this protein, Hcr1, has been implicated in stringent AUG recognition as well as in controlling translation termination and stop codon readthrough. Using a reconstituted mammalian in vitro translation system, we showed that the human protein eIF3j is also important for translation termination. We showed that eIF3j stimulates peptid ...[more]

PMID: 34591963

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EIF3j facilitates loading of release factors into the ribosome.

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eIF3j facilitates loading of release factors into the ribosome.

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