A CRISPR/Cas9 method facilitates efficient oligo-mediated gene editing in Debaryomyces hansenii.

ABSTRACT: Halophilic and osmotolerant yeast Debaryomyces hansenii has a high potential for cell factory applications due to its resistance to harsh environmental factors and compatibility with a wide substrate range. However, currently available genetic techniques do not allow the full potential of D. hansenii as a cell factory to be harnessed. Moreover, most of the currently available tools rely on the use of auxotrophic markers that are not suitable in wild-type prototrophic strains. In addition, the preferred non-homologous end-joining (NHEJ) DNA damage repair mechanism poses further challenges when precise gene targeting is required. In this study, we present a novel plasmid-based CRISPR^CUG/Cas9 method for easy and efficient gene editing of the prototrophic strains of

SUBMITTER: Strucko T 

PROVIDER: S-EPMC8566172 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature