Role of TLR4 in Neutrophil Dynamics and Functions: Contribution to Stroke Pathophysiology.

ABSTRACT:

Background and purpose

The immune response subsequent to an ischemic stroke is a crucial factor in its physiopathology and outcome. It is known that TLR4 is implicated in brain damage and inflammation after stroke and that TLR4 absence induces neutrophil reprogramming toward a protective phenotype in brain ischemia, but the mechanisms remain unknown. We therefore asked how the lack of TLR4 modifies neutrophil function and their contribution to the inflammatory process.

Methods

In order to assess the role of the neutrophilic TLR4 after stroke, mice that do not express TLR4 in myeloid cells (TLR4^loxP/Lyz-cre) and its respective controls (TLR4^loxP/loxP) were used. Focal cerebral ischemia was induced by occlusion of the middle cerebral artery and infarct si

SUBMITTER: Duran-Laforet V 

PROVIDER: S-EPMC8566541 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature