Characterization of NAD⁺/NADP⁺-Specific Isocitrate Dehydrogenases From Oleaginous Fungus Mortierella alpina Involved in Lipid Accumulation.

ABSTRACT: Mortierella alpina has a strong capacity for lipid accumulation. Isocitrate dehydrogenase (IDH) plays an important role in affecting the flow of intracellular carbon sources and reducing power NADPH for lipid biosynthesis. In this study, the effect of various IDHs (NAD⁺- and NADP⁺-specific) in M. alpina on the lipid accumulation was investigated through homologous overexpression. The results showed that the transcription level and enzyme activity of the IDHs from M. alpina (MaIDHs) in homologous overexpressing strains were higher than those of the control strain, but that their biomass was not significantly different. Among the various NAD⁺-specific MaIDH1/2/3 overexpression, NAD⁺-MaIDH3 reduced total lipid content by 12.5%,

SUBMITTER: Tang X 

PROVIDER: S-EPMC8566678 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature