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Stilbene compounds are specific inhibitors of the superoxide anion generation catalyzed by xanthine oxidase.

ABSTRACT: The inhibitory effect of xanthine oxidase (XO) reactions with stilbene compounds, 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging by stilbene compounds and superoxide anion (O₂-) scavenging activity were examined. The inhibition of the O₂- generation catalyzed by XO by stilbene compounds is stronger than the effect on uric acid formation. The suppression of the O₂- generation with resveratrol was diminished by the addition of flavin adenine dinucleotide (FAD). The water-solubility and visible spectra (VIS) of the stilbene compounds in the presence of water-soluble flavin compounds indicated a π-π interaction between the stilbene compounds and the isoalloxazine in flavin compounds. These results indicate that stilbene compounds specifically bind the FAD site in XO so as to inhibit the O₂- generation. In the case of piceatannol, it is deduced that the suppression of O₂- generation is induced by this specific binding to the FAD site and the subsequent reduction of XO.

SUBMITTER: Masuoka N

PROVIDER: S-EPMC8566757 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature

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Stilbene compounds are specific inhibitors of the superoxide anion generation catalyzed by xanthine oxidase.

Masuoka Noriyoshi N

Food chemistry: X 20211028

The inhibitory effect of xanthine oxidase (XO) reactions with stilbene compounds, 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging by stilbene compounds and superoxide anion (O2-) scavenging activity were examined. The inhibition of the O2- generation catalyzed by XO by stilbene compounds is stronger than the effect on uric acid formation. The suppression of the O2- generation with resveratrol was diminished by the addition of flavin adenine dinucleotide (FAD). Th ...[more]

PMID: 34761201

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