Rapid Detection and Quantification of Plant Innate Immunity Response Using Raman Spectroscopy.

ABSTRACT: We have developed a rapid Raman spectroscopy-based method for the detection and quantification of early innate immunity responses in Arabidopsis and Choy Sum plants. Arabidopsis plants challenged with flg22 and elf18 elicitors could be differentiated from mock-treated plants by their Raman spectral fingerprints. From the difference Raman spectrum and the value of p at each Raman shift, we derived the Elicitor Response Index (ERI) as a quantitative measure of the response whereby a higher ERI value indicates a more significant elicitor-induced immune response. Among various Raman spectral bands contributing toward the ERI value, the most significant changes were observed in those associated with carotenoids and proteins. To validate these results, we investigated several characterize

SUBMITTER: Chung PJ 

PROVIDER: S-EPMC8566886 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature