Decellularized Whole-Organ Pre-vascularization: A Novel Approach for Organogenesis.

ABSTRACT: Introduction: Whole-organ decellularization is an attractive approach for three-dimensional (3D) organ engineering. However, progress with this approach is hindered by intra-vascular blood coagulation that occurs after in vivo implantation of the re-cellularized scaffold, resulting in a short-term graft survival. In this study, we explored an alternative approach for 3D organ engineering through an axial pre-vascularization approach and examined its suitability for pancreatic islet transplantation. Methods: Whole livers from male Lewis rats were decellularized through sequential arterial perfusion of detergents. The decellularized liver scaffold was implanted into Lewis rats, and an arteriovenous bundle was passed through the scaffold. At the time of implantation, fres

SUBMITTER: Fathi I 

PROVIDER: S-EPMC8567193 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature