Noninvasive Tracking of Natural Killer Cells Using Gold Nanoparticles.

ABSTRACT: Natural killer (NK)-cell-based immunotherapy is emerging as an attractive approach for cancer treatment. However, to facilitate and expedite clinical implementation, important questions must be answered regarding the in vivo functionality and trafficking patterns of the transferred cells. We have recently developed a noninvasive cell-tracking technique, based on gold nanoparticles (GNPs) as cell-labeling and contrast agents for whole-body computed tomography (CT) imaging. Herein, we report the implementation of this technique for longitudinal and quantitative tracking of NK cell kinetics, the migration and biodistribution in tumor-bearing mice. NK cells were successfully labeled with GNPs, without impairing their biological function, as assessed both in vitro, by cytokine rel

SUBMITTER: Shamalov K 

PROVIDER: S-EPMC8567284 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature