Development of a novel real-time polymerase chain reaction assay for the sensitive detection of Schistosoma japonicum in human stool.

ABSTRACT:

Background

Elimination and control of Schistosoma japonicum, the most virulent of the schistosomiasis-causing blood flukes, requires the development of sensitive and specific diagnostic tools capable of providing an accurate measurement of the infection prevalence in endemic areas. Typically, detection of S. japonicum has occurred using the Kato-Katz technique, but this methodology, which requires skilled microscopists, has been shown to radically underestimate levels of infection. With the ever-improving capabilities of next-generation sequencing and bioinformatic analysis tools, identification of satellite sequences and other highly repetitive genomic elements for use as real-time PCR diagnostic targets is becoming increasingly common. Assays developed using these targets have th

SUBMITTER: Halili S 

PROVIDER: S-EPMC8568117 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature