Molecular study of Nucleophosmin 1(NPM1) gene in acute myeloid leukemia in Kurdish population.

ABSTRACT:

Background

In patients with Acute Myeloid Leukemia (AML) the most frequent acquired molecular abnormalities and important prognostic indicators is nucleophosmin-1 (NPM1) mutations. Our study aims was molecular study of Nucleophosmin -1 gene in Acute Myeloid Leukemia in Kurdish population.

Patients &methods

A total of 50 patients with AML, (36) of them attended Nanakaly Hospital and (14) attended Hiwa Hospital and 30 healthy subjects as control were selected randomly, all were matched of age and gender. Polymerase chain reaction (PCR) was used for detection of NPM1 gene mutation. Three samples of PCR product for NPM1 gene mutations were sequenced, and mutations were determined by comparison with the normal NPM1 sequence NCBI (GenBank accession number NM_002520).

Results

SUBMITTER: Othman GO 

PROVIDER: S-EPMC8568245 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature