High-throughput screening of α-chiral-primary amines to determine yield and enantiomeric excess.

ABSTRACT: A novel screening protocol was developed using a combination of a fluorescent indicator displacement assay and a circular dichroism (CD) active Fe(II) complex to determine concentration and enantiomeric excess (ee) of α-chiral amines, respectively. The analyte concentration is quantified with a pre-formed non-fluorescent imine, where transimination with the chiral amine results in displacement of the fluorophore 2-naphthylamine. After discerning the concentration of amine via fluorescence in a wellplate reader, the analyte is then incorporated into a three-component octahedral Fe(II) assembly for ee determination using an EKKO CD plate-reader. With these two assays, both the ee and yield of asymmetric transformations of 192 samples could be determined with acceptable e

SUBMITTER: Moor SR 

PROVIDER: S-EPMC8568316 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature