Listeria monocytogenes 10403S Alternative Sigma-54 Factor σ^L Has a Negative Role on Survival Ability Under Bile Exposure.

ABSTRACT: Listeria monocytogenes is a Gram-positive bacterium causing listeriosis in animals and humans. To initiate a foodborne infection, L. monocytogenes has to pass through the host gastrointestinal tract (GIT). In this study, we evaluated survival abilities of L. monocytogenes 10403S wild type (WT) and its isogenic mutants in alternative sigma (σ) factor genes (i.e., sigB, sigC, sigH, and sigL) under simulated gastric, duodenal, and bile fluids. Within 10min of exposures, only bile fluid was able to significantly reduce survival ability of L. monocytogenes WT by 2 logs CFU/ml. Loss of sigL showed the greatest bile resistance among 16 strains tested, p<0.0001, (i.e., WT, four single alternative σ factor mutants, six double mut

SUBMITTER: Boonmee A 

PROVIDER: S-EPMC8568364 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature