The Mechanism Exploration of Follicular Fluids on Granulose Cell Apoptosis in Endometriosis-Associated Infertility.

ABSTRACT:

Objective

To explore the mechanisms of follicular fluids (FFs) on granulose cell (GC) apoptosis in endometriosis-associated infertility.

Materials and methods

60 infertile women were enrolled. The FFs from 30 endometriosis-associated infertility (EI) patients were collected and processed by ELISA hormone assay and proteomic profiling. The ovary GCs collected from 30 tubal-associated infertility (TI) patients were cultured in follicular fluids of endometriosis-associated infertility patients (EI-FFs), and the apoptosis mechanisms were explored by flow cytometry assay, real-time PCR, Western blotting, and protein-protein interaction (PPI) network analysis.

Results

Our results showed that the expression of 22 specific proteins was significantly different in the FFs from

SUBMITTER: Chen L 

PROVIDER: S-EPMC8568523 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature