CRAC Channel Controls the Differentiation of Pathogenic B Cells in Lupus Nephritis.

ABSTRACT: Store-operated Ca²⁺ release-activated Ca²⁺ (CRAC) channel is the main Ca²⁺ influx pathway in lymphocytes and is essential for immune response. Lupus nephritis (LN) is an autoimmune disease characterized by the production of autoantibodies due to widespread loss of immune tolerance. In this study, RNA-seq analysis revealed that calcium transmembrane transport and calcium channel activity were enhanced in naive B cells from patients with LN. The increased expression of ORAI1, ORAI2, and STIM2 in naive B cells from patients with LN was confirmed by flow cytometry and Western blot, implying a role of CRAC channel in B-cell dysregulation in LN. For in vitro study, CRAC channel inhibition by YM-58483 or downregulation by ORAI1-specific small-interfering RN

SUBMITTER: Li X 

PROVIDER: S-EPMC8569388 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature