Effects of Wild Yam Root (Dioscorea villosa) Extract on the Gene Expression Profile of Triple-negative Breast Cancer Cells.

ABSTRACT:

Background/aim

Wild yam extract [Dioscorea villosa, (WYE)] is consistently lethal at low IC₅₀s across diverse cancer-lines in vitro. Unlike traditional anti-cancer botanicals, WYE contains detergent saponins which reduce oil-water interfacial tensions causing disintegration of lipid membranes and causing cell lysis, creating an interfering variable. Here, we evaluate WYE at sub-lethal concentrations in MDA-MB-231 triple-negative breast cancer (TNBC) cells.

Materials and methods

Quantification of saponins, membrane potential, lytic death and sub-lethal WYE changes in whole transcriptomic (WT) mRNA, miRNAs and biological parameters were evaluated.

Results

WYE caused 346 differentially expressed genes (DEGs) out of 48,226 transcripts tested; where up-regulated

SUBMITTER: Mazzio E 

PROVIDER: S-EPMC8569819 | biostudies-literature | 2021 Nov-Dec

REPOSITORIES: biostudies-literature