Differential p16 expression levels in the liver, hepatocytes and hepatocellular cell lines.

ABSTRACT:

Background

One of the most frequently deleted genes in cancer is CDKN2A encoding p16. This protein is often overexpressed in senescent cells, while its suppression can bypass the oncogene-induced senescence to enable transformation and tumorigenesis. The roles of the protein p16 are recently being expanded from the cell cycle progression regulator to the cellular regulator interacting in several different pathways. Yet data on its liver and liver cells' expression are inconclusive.

Methods

The expression of the p16 gene in liver and liver cells was determined by RT-qPCR and compared to its protein amounts by western blotting.

Results

p16 is expressed at low levels in the liver and rat hepatocytes. Its expression varies from none to the considerabl

SUBMITTER: Kramar B 

PROVIDER: S-EPMC8570159 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature