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Function analysis and stress-mediated cis-element identification in the promoter region of VqMYB15.


ABSTRACT: The transcription factor MYB15 plays an important role in grape basal immunity, and its promoter can be used as a potential target in resistance breeding. However, the regulatory mechanisms of cis-elements in its promoter region under a variety of stresses remain unclear. In this study, we identified some putative cis-regulatory elements present upstream of MYB15 in Vitis quinquangularis Shanyang (pVqMYB15_SY) and subsequently characterized the function of these elements using reporter assays. Our results showed that TCA-elements 1 and 2, ABRE, MYC and 3-AF1 binding site 1 are key cis-regulatory elements in pVqMYB15_SY and play important roles in plant bio/abiotic stress resistance.

SUBMITTER: Li R 

PROVIDER: S-EPMC8570707 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature

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Function analysis and stress-mediated <i>cis</i>-element identification in the promoter region of <i>VqMYB15</i>.

Li Ruixiang R   Zhu Fanding F   Duan Dong D  

Plant signaling & behavior 20200530 7


The transcription factor <i>MYB15</i> plays an important role in grape basal immunity, and its promoter can be used as a potential target in resistance breeding. However, the regulatory mechanisms of <i>cis</i>-elements in its promoter region under a variety of stresses remain unclear. In this study, we identified some putative <i>cis</i>-regulatory elements present upstream of <i>MYB15</i> in <i>Vitis quinquangularis</i> Shanyang (<i>pVqMYB15</i>_SY) and subsequently characterized the function  ...[more]

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