Ceramide synthase 6 mediates sex-specific metabolic response to dietary folic acid in mice.

ABSTRACT: Folic acid-fortified foods and multi-vitamin supplements containing folic acid (FA) are widely used around the world, but the exact mechanisms/metabolic effects of FA are not precisely identified. We have demonstrated that Ceramide Synthase 6 (CerS6) and C_16:0-ceramide mediate response to folate stress in cultured cells. Here we investigated the dietary FA effects on mouse liver metabolome, with a specific focus on sphingolipids, CerS6 and C_16:0-ceramide. Wild-type and CerS6^-/- mice were fed FA-deficient, control, or FA over-supplemented diets for 4 weeks. After dietary treatment, liver concentrations of ceramides, sphingomyelins and hexosylceramides were measured by LC-MS/MS and complemented by untargeted metabolomic characterization of mouse livers. Our s

SUBMITTER: Barron K 

PROVIDER: S-EPMC8571033 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature