Sex Differences in the Role of CNIH3 on Spatial Memory and Synaptic Plasticity.

ABSTRACT:

Background

CNIH3 is an AMPA receptor (AMPAR) auxiliary protein prominently expressed in the dorsal hippocampus (dHPC), a region that plays a critical role in spatial memory and synaptic plasticity. However, the effects of CNIH3 on AMPAR-dependent synaptic function and behavior have not been investigated.

Methods

We assessed a gain-of-function model of Cnih3 overexpression in the dHPC and generated and characterized a line of Cnih3^-/- C57BL/6 mice. We assessed spatial memory through behavioral assays, protein levels of AMPAR subunits and synaptic proteins by immunoblotting, and long-term potentiation in electrophysiological recordings. We also utilized a super-resolution imaging workflow, SEQUIN (Synaptic Evaluation and Quantification by Imaging of Nanostructure),

SUBMITTER: Frye HE 

PROVIDER: S-EPMC8571071 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature