Ontology highlight
ABSTRACT:
SUBMITTER: Lei KH
PROVIDER: S-EPMC8571288 | biostudies-literature | 2021 Nov
REPOSITORIES: biostudies-literature
Lei Kai-Hang KH Yang Han-Lin HL Chang Hao-Yen HY Yeh Hsin-Yi HY Nguyen Dinh Duc DD Lee Tzu-Yu TY Lyu Xinxing X Chastain Megan M Chai Weihang W Li Hung-Wen HW Chi Peter P
Nature communications 20211105 1
Replication stress causes replication fork stalling, resulting in an accumulation of single-stranded DNA (ssDNA). Replication protein A (RPA) and CTC1-STN1-TEN1 (CST) complex bind ssDNA and are found at stalled forks, where they regulate RAD51 recruitment and foci formation in vivo. Here, we investigate crosstalk between RPA, CST, and RAD51. We show that CST and RPA localize in close proximity in cells. Although CST stably binds to ssDNA with a high affinity at low ionic strength, the interactio ...[more]