Pseudoknot length modulates the folding, conformational dynamics, and robustness of Xrn1 resistance of flaviviral xrRNAs.

ABSTRACT: To understand how RNA dynamics is regulated and connected to its function, we investigate the folding, conformational dynamics and robustness of Xrn1 resistance of a set of flaviviral xrRNAs using SAXS, smFRET and in vitro enzymatic assays. Flaviviral xrRNAs form discrete ring-like 3D structures, in which the length of a conserved long-range pseudoknot (PK2) ranges from 2 bp to 7 bp. We find that xrRNAs' folding, conformational dynamics and Xrn1 resistance are strongly correlated and highly Mg²⁺-dependent, furthermore, the Mg²⁺-dependence is modulated by PK2 length variations. xrRNAs with long PK2 require less Mg²⁺ to stabilize their folding, exhibit reduced conformational dynamics and strong Xrn1 resistance even at low Mg²⁺, and tolerate mutatio

SUBMITTER: Niu X 

PROVIDER: S-EPMC8571300 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature