Massively parallel interrogation of protein fragment secretability using SECRiFY reveals features influencing secretory system transit.

ABSTRACT: While transcriptome- and proteome-wide technologies to assess processes in protein biogenesis are now widely available, we still lack global approaches to assay post-ribosomal biogenesis events, in particular those occurring in the eukaryotic secretory system. We here develop a method, SECRiFY, to simultaneously assess the secretability of >10⁵ protein fragments by two yeast species, S. cerevisiae and P. pastoris, using custom fragment libraries, surface display and a sequencing-based readout. Screening human proteome fragments with a median size of 50-100 amino acids, we generate datasets that enable datamining into protein features underlying secretability, revealing a striking role for intrinsic disorder and chain flexibility. The SECRiFY methodology generates sufficient amou

SUBMITTER: Boone M 

PROVIDER: S-EPMC8571348 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature