The contribution of TFPIα to the hemostatic response to injury in mice.

ABSTRACT:

Background

Tissue factor pathway inhibitor (TFPI) is an essential regulator of coagulation, limiting thrombin generation and preventing thrombosis. In humans and mice, TFPIα is the sole isoform present in platelets.

Objective

Here, we asked whether TFPIα, because of its release from platelets at sites of injury, has a unique role in limiting the hemostatic response.

Methods

TFPIα-mutant (Tfpi^Δα/Δα ) mice were generated by introducing a stop codon in the C-terminus. Platelet accumulation, platelet activation, and fibrin accumulation were measured following penetrating injuries in the jugular vein and cremaster muscle arterioles, and imaged by fluorescence and scanning electron microscopy. Time to bleeding cessation was recorded in the jugular vein studies.

SUBMITTER: Marar TT 

PROVIDER: S-EPMC8571650 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature