Detection of glucose-derived D- and L-lactate in cancer cells by the use of a chiral NMR shift reagent.

ABSTRACT:

Background

Excessive lactate production, a hallmark of cancer, is largely formed by the reduction of pyruvate via lactate dehydrogenase (LDH) to L-lactate. Although D-lactate can also be produced from glucose via the methylglyoxal pathway in small amounts, less is known about the amount of D-lactate produced in cancer cells. Since the stereoisomers of lactate cannot be distinguished by conventional ¹H NMR spectroscopy, a chiral NMR shift reagent was used to fully resolve the ¹H NMR resonances of D- and L-lactate.

Methods

The production of L-lactate from glucose and D-lactate from methylglyoxal was first demonstrated in freshly isolated red blood cells using the chiral NMR shift reagent, YbDO3A-trisamide. Then, two different cell lines with high GLO1 exp

SUBMITTER: Suh EH 

PROVIDER: S-EPMC8571830 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature