An Atlas of Heparan Sulfate Proteoglycans in the Postnatal Rat Lens.

ABSTRACT:

Purpose

The arrangement of lens cells is regulated by ocular growth factors. Although the effects of these inductive molecules on lens cell behavior (proliferation, survival, and fiber differentiation) are well-characterized, the precise mechanisms underlying the regulation of growth factor-mediated signaling in lens remains elusive. Increasing evidence highlights the importance of heparan sulfate proteoglycans (HSPGs) for the signaling regulation of growth factors; however, the identity of the different lens HSPGs and the specific roles they play in lens biology are still unknown.

Methods

Semiquantitative real-time (RT)-PCR and immunolabeling were used to characterize the spatial distribution of all known HSPG core proteins and their associated glycosaminoglycans (heparan a

SUBMITTER: Wishart TFL 

PROVIDER: S-EPMC8572486 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature