High-throughput next-generation sequencing for identifying pathogens during early-stage post-lung transplantation.

ABSTRACT:

Background

High-throughput next-generation sequencing (HT-NGS) has the potential to detect a large variety of pathogens; however, the application of HT-NGS in lung transplant (LTx) recipients remains limited. We aimed to evaluate the value of HT-NGS for pathogen detection and diagnosis of pulmonary infection during early-stage post-lung transplantation.

Methods

In this retrospective study, we enrolled 51 LTx recipients who underwent lung transplantation between January 2020 and December 2020. Bronchoalveolar lavage fluid (BALF) samples were collected for the detection of pathogens using both HT-NGS and conventional microbiological testing. The detection of pathogens and diagnostic performance of HT-NGS were compared with that of conventional methods.

Results

HT-NGS pr

SUBMITTER: Lian QY 

PROVIDER: S-EPMC8572506 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature